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重組Anti-Ki67抗體[SP6],兔單克隆抗體[SP6] to Ki67,Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply.
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Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
WB: HeLa cell lysate. IHC-P: Human tonsil, rat and mouse spleen tissues. ICC/IF: HeLa and HAP1 cells. Flow Cyt (intra): HAP1 cells.
ab16667 was switched from a hybridoma to recombinant production method on 24th October 2019.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information see here.Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Abpromise™承諾保證使用ab16667于以下的經測試應用
“應用說明"部分 下顯示的僅為推薦的起始稀釋度；實際最佳的稀釋度/濃度應由使用者檢定。
| 應用 | Ab評論 | 說明 |
|---|---|---|
| Flow Cyt (Intra) | 1/1000. ab172730, Rabbit monoclonal isotype, is suitable for use as an isotype control with this antibody. | |
| IHC-P | (62) | 1/200. Antigen retrieval: Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). Primary antibody condition: primary antibody incubation overnight at +4°C is recommended. |
| WB | (3) | Use at an assay dependent concentration. Predicted molecular weight: 358 kDa. |
| mIHC | Use at an assay dependent concentration. | |
| ICC/IF | (22) | 1/250. If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min. |
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